Objective To explore the effects of brain derived neurotrophic factor (BDNF) on proliferation and differentiation of child preadipocytes,and provide guidance for the treatment of childhood obesity. Methods Adipose tissue were collected from normal and obese children,real-time qPCR and western blotting were used respectively for measuring the expression of mRNA and protein of BDNF,C1q/TNF-related protein-13(CTRP13) and IL-6;Preadipocytes were isolated from adipose tissue and cultured with different concentrations of BDNF and BDNF siRNA respectively,the level of preadipocytes' proliferation was determined by MTT and the differentiation by oilred O,triglyceride (TG) assay kits was used for detecting the intracellular lipogenesis,the levels of PPARγ,aP2,CTRP13 and IL-6 were determined by western blotting. Results The obesity children showed higher expression of IL-6 in adipose tissue compared to control group,while lower expression of CTRP13 and BDNF in obesity children.BDNF had no effects for proliferation of children preadipocyte; BDNF promoted the expression of CTRP13 in children preadipocytes,while inhabited IL-6 and differentiation of preadipocytes.Moreover,BDNF siRNA showed the opposite effect with BDNF. Conclusion IL-6 was overexpression in obesity children,but BDNF and CTRP13 are lower in obesity children compared to the normal,BDNF can inhibit the differentiation of preadipocytes.
Key words
obesity of children /
brain derived neurotrophic factor /
adipogenic differentiation /
C1q/TNF-related protein-13 /
interleukin-6
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