Objective To investigate the etiology of infectious diarrhea in Yinchuan children and provide the basis for clinical rational drug use. Methods During April 2012 to April 2013 the Ningxia People's Hospital outpatient and hospitalized children with diarrhea patients initial diagnosis had the fresh fecal bacterial culture,and the resulting samples were tested by RT-PCR technology for rotavirus (HRV),norovirus (NORV),bar-like viruses (SPAV),astrovirus (ASTV) and enteric adenovirus (EADV). Results A total of 286 stool samples were detected,208 cases found pathogen with detection rate of 72.73%.Virus detection,the positive rate of 172 cases was 60.14%,which HRV 84 cases (29.34%),NORV 62 cases (21.77%),SPAV 14 cases (4.90%),ASTV 27 cases (9.44%),EADV 21 cases (7.34%).The detection rate of bacterial culture pathogens was 12.58%(36 cases).Salmonella infection was more common.Mixed infection was 36 cases (12.58%). Conclusion Yinchuan infectious diarrhea in children is connected with viral infections,HRV,NORV as the main pathogens.
Key words
diarrhea /
pathogen detection /
reverse transcription-polymerase chain reaction
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