Objective To investigate the effect of various ischemic methods on the pathological change of brain and ocular ischemic syndrome in the Sprague-Dawley (SD) neonatal rats with periventricular leukomalacia(PLV). Methods Two-old-day SD neonatal rats were randomly divided into three groups:the unilatera group, the bilateral group, the sham operation group.Unilateral group by ligation of right common carotid artery, bilateral group were treated with the bilateral common carotid artery-ligation, both of them followed by 8%O2+92%N2 for 1 hour.The pathological changes in the brain were examined by optical microscope, and the ocular ischemic syndrome were monitored dynamic in the three groups. Results 1)Eye lesions:on day 1 after operation, the eyes were displaying purple swelling in the bilateral group; at the 11th and 18th days after operation, there were various degrees of eye lesions in bilateral group, such as the palpebral fissure narrowed, atrophy of eyeball, single or both blind, lens opacity and cataracts were confirmed by slit lamp, there were no eye lesions in the unilatera group and the sham operation group.2)Pathological changes of brain:On the 4th day after operation, neurocytes loose, edema, necrosis, microglia proliferation could be observed, and the bilateral group was more serious than the unilateral group, and even the liquefactive necrosis could be observed in the bilateral group;On the 11th and the 18th day after operation, the periventricular white matter reduction and the local malacia around the periventricular area were observed in the bilateral group and the unilateral group, the damage of brain in the bilateral group was serious than the unilatera group, there were no changes in the sham operation group. Conclusions This study suggests that the PVL animal model of two-day-old neonatal rat is successfully established by the ligation of unilateral common carotid artery or bilateral common carotid artery, and then exposure hypoxia for 1 hour; and also accompany with ocular ischemic syndrome by the ligation of bilateral common carotid artery in 2-day-old neonatal rat.
Key words
periventricular leukomalacia /
animal model /
neonatal rat /
ocular ischemic syndrome
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