目的 比较ω-3PUFAs、ω-6PUFAs和ω-9MUFAs三种脂肪乳剂对脂多糖(lipid emulsions, LPS)诱导的急性肺损伤大鼠IL-6分泌的影响。方法 100只大鼠按随机数字分为Control组、LPS组、Intra组、Clino组和Omega组。分别采用生理盐水或不同种类脂肪乳剂处理7 d;并均在取标本前8 h时于气管内滴入生理盐水或LPS, 建立ALI大鼠模型;观察各组大鼠肺组织的病理改变、凋亡指数, 测定IL-6mRNA变化以及IL-6蛋白浓度。结果 1)各组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血;2)Clino组和Omega组血清中IL-6mRNA表达较Intra组、LPS组均明显降低;BALF中Clino组和Omega组IL-6蛋白水平明显低于Intra组、LPS组, 差异具有统计学意义(P均＜0.005)。而Clino组和Omega组两组IL-6mRNA表达及其蛋白水平差异均无统计学意义(P均＞0.005)。3)Clino组和Omega组凋亡系数均低于LPS组和Intra组(P均<0.005);Clino组和Omega组AI值差异无统计学意义(P＞0.005)。结论 Clinoleic和Omegaven可通过降低IL-6而减轻ALI大鼠炎症反应, 并能减轻肺泡上皮细胞凋亡。
Abstract
Objective To compare the effects of three kinds of lipid emulsions to IL-6, and alveolar epithelial cell apoptosis of lipid emulsions (LPS)-induced rat lung of inflammatory. Methods One hundered young Sprague-Dawley rats were divided into control group, LPS group, intra group, Clino group and Omega group and then used normal saline or different fat emulsions for seven days.LPS was installed in order to establish the rat model of acute lung injury (ALI).Those rats were intratracheally instilled with normal saline or LPS 8 hours before sacrifice.Each lung tissue section's pathologic changes was observed, and apoptosis index(AI) was detected.Expression level of IL-6 mRNA was examined by RT-PCR, meanwhile the concentrations of IL-6 in BALF were measured by enzyme-linked immunospecific assay (ELISA). Results 1)The expressions of IL-6 mRNA in serum and the level of IL-6 in BALF of LPS groups and intra group were significantaly elevated than clino group and omega group (both P<0.005), but it was no significant difference(P＞0.005)when Clino group compared with Omega group.2)pulmonary histopathology:there were demonstrated significant inflammatory in light microscopy of HE-stained lungs tissues of rat, and they were showing inflammatory cells infiltrating around the bronchus and the vascular, even bleeding.3)apoptosis results:compared with LPS group and intra group, the AI of Clino group and Omega group significantly reduced(both P<0.005), which was no significant difference between each other(P＞0.005). Conclusion 20% clinoleic and 10% omegaven can relieve inflammation by downregulating the concentrations of IL-6, and reduce the alveolar epithelial cell apoptosis in rats with acute lung injury.
关键词
脂多糖 /
急性肺损伤 /
白介素6
Key words
lipid emulsions /
acute lung injury /
IL-6
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基金
浙江省医学重点扶植学科“临床营养学(2007-F03)”
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