目的 探讨宫内生长受限(intrauterine growth restriction，IUGR)胎鼠脑组织中PirB的表达情况及孕鼠补充牛磺酸对其表达的影响。方法 采用全程低蛋白饮食法建立IUGR胎鼠模型，将18只孕鼠随机分为对照组、IUGR模型组(IUGR组)、IUGR+孕鼠补充牛磺酸组(牛磺酸组)，每组6只。每窝随机抽取3只胎鼠断头取脑。应用免疫组化法检测各组胎鼠脑组织每高倍视野PirB阳性细胞数，Western Blot 法检测各组胎鼠脑组织中PirB蛋白表达情况，实时荧光定量PCR(Real-time PCR，RT-PCR)法检测各组胎鼠脑组织中PirB mRNA的表达情况。结果 对照组、IUGR组与牛磺酸组: 1)免疫组化法检测三组中PirB阳性细胞数分别为(18.40±1.52)、(66.17±3.66)、(21.17±2.71)个/高倍视野;2)Western Blot 法检测三组中PirB蛋白半定量分析结果PirB/β-actin分别为0.05、0.31、0.09; 3)RT-PCR法检测三组中PirB-mRNA表达水平的2-ΔΔCT值分别为1(0.87，1.15)、0.08(0.06，0.11)、1.22(0.97，1.55)。对照组、牛磺酸组与IUGR组比较，PirB阳性细胞数均低于IUGR组，PirB mRNA和蛋白的表达均低于IUGR组，牛磺酸组与IUGR组比较差异均有统计学意义(P<0.05)。结论 本研究结果显示孕鼠补充牛磺酸后IUGR胎鼠脑组织PirB的表达减少，提示牛磺酸可能通过减少或抑制PirB的表达而发挥对IUGR胎鼠脑损伤的保护作用。
Abstract
Objective To explore the expression of paired immuonglobin-like receptor B(PirB) in fetal rat brain tissue with intrauterine growth restriction (IUGR) and the influence of antenatal taurine on its expression. Methods Eighteen pregnant rats were randomly divided into 3 groups:normal control group,IUGR models group(IUGR group) and the IUGR +antenatal taurine supplement group(taurine group)(n=6.IUGR models were induced by low protein diet throughout gestation period.Three fetal rats were randomly selected from each nest and were sacrificed to obtain the brains.The PirB positive cell counts were detected by immunohistochemistry,the PirB protein contents were deteceted by Western Blot and the level of mRNA expressions of PirB gene were detected by Real time-PCR. Results Control group,IUGR group,taurine group:1)The PirB positive cell counts in the three groups were respectively 18.40±1.52,66.17±3.66,21.17±2.71; 2)The PirB protein semi-quantitative analysis results respectively 0.05,0.31,0.09; 3)The level of PirB mRNA 2-ΔΔCT numerical respectively 1(0.87,1.15),0.08(0.06,0.11),1.22(0.97,1.55).Compared control group and taurine group with IUGR group,PirB positive cell counts were lower than that of IUGR group,PirB mRNA and protein expression were lower than that of IUGR group.The differences of comparing IUGR group with taurine group were statistically significant(P<0.05). Conclusion The results of this study show that the expression of PirB in fetal rat brain tissues is higher in IUGR groups than that in controls while antenatal taurine can significantly decrease its expression,which suggests that antenatal taurine may play a protecting role by inhibiting the expression of PirB in fetal brain tissues.
关键词
宫内生长受限 /
胎鼠 /
牛磺酸 /
配对免疫球蛋白样受体B
Key words
intrauterine growth restriction /
fetal rat /
taurine /
paired immuonglobin-like receptor B
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基金
国家自然科学基金资助项目(81170577)
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