目的 探讨哮喘患儿诱导痰中嗜酸性粒细胞与哮喘发病的关系, 血清中IL-4和IFN-γ水平变化在哮喘患儿中的意义, 完善儿童哮喘临床诊断方法。方法 选取2011年2月-2012年6月在本院儿科住院的急性发作期哮喘患儿69例, 及同期在院体检的健康者42例为对照, 对两组受试者进行肺功能测试、痰液的诱导和处理, 计数嗜酸性粒细胞百分率、采用酶联免疫吸附实验法(ELISA)检测血清中IL-4和IFN-γ水平。结果 与正常对照组相比, 哮喘组FEV1%和FEV1占预计值%等肺功能指标明显降低(P<0.05), 而嗜酸性粒细胞百分率明显提高(P=0.009);哮喘组IL-4水平明显高于对照组(P=0.012), IFN-γ水平明显低于对照组(P=0.020), 差异均有统计学意义(P<0.05)。结论 嗜酸性粒细胞增加、IL-4水平明显升高、IFN-γ水平降低与儿童支气管哮喘的发生与发展有密切关系, 可以结合肺功能指标更好地完善儿童哮喘的临床诊断方法。

Objective To investigate the relation between EOS and asthma;To evaluate the alteration and significance of serum IL-4 and IFN-γ in bronchial asthma. Methods Forced expiratory volume in one second (FEV1) was measured in all patients as a percent of predicted value; The use of ultrasonic inhalation of 3% to 5% hypertonic saline induced EOS percentage ;The serum levels of IL-4 and IFN-γ were measured by ELISA in 69 cases of asthma at stage of episode, and 42 cases healthy people. Results The levels of EOS and IL-4 in bronchial asthma patients were significantly higher than of normal control group (P=0.009;P=0.012);while the level of IFN-γ in bronchial asthma patients were significantly lower than of normal control group(P=0.020). Conclusion Augmentation of EOS, IL-4 and IFN-γ had close correlation with bronchial asthma, to improve the clinical diagnosis of asthma.