目的 分析ω-3PUFAs对脂多糖诱导的急性肺损伤大鼠(acute lung injury, ALI)TNF-α、IL-1β和IL-6分泌的影响。方法 60只Sprague-Dawley幼年大鼠按随机数字分为Control组(生理盐水+生理盐水)、LPS组(生理盐水+脂多糖)和Omega(10%脂肪乳尤文+脂多糖)组。各组分别采用生理盐水或尤文脂肪乳剂处理7 d;并均在取标本前8 h时于气管内滴入生理盐水或脂多糖, 建立ALI大鼠模型;观察各组大鼠肺组织的病理改变, 测定肺泡灌洗液TNF-α、IL-1β、IL-6的表达。结果 1)ALI模型组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血;2)ALI模型组肺系数、病理评分均高于Control组(P均＜0.05);3)BALF中Omega组TNF-α、IL-1β和IL-6蛋白水平低于LPS组, 差异具有统计学意义(P均＜0.05)。结论 ω-3PUFAs能够通过降低TNF-α、IL-1β和IL-6的分泌而减轻ALI大鼠炎症反应, 减轻肺部损伤。
Abstract
Objective To investigate the effects of ω-3 polyunsaturated fatty acids (ω-3PUFAs) to LPS-induced rat lung(ALI) of inflammatory:TNF-α, IL-1 β and IL-6. Methods Sixty young SD rats were divided into control group, LPS group and omega group and then used normal saline or different fat emulsions for seven days.The rat model of ALI was established by instill LPS.Those groups were sacrificed at 8 hours after intratracheal introduction of LPS.Each lung tissue section's pathologic changes was observed, meanwhile the concentrations of TNF-α, IL-1 β and IL-6 in BALF were measured by enzyme-linked immunospecific assay (ELISA). Results 1)Pulmonary histopathology:ALI modle groups showed inflammatory cells infiltrating around the bronchus and the vascular, even bleeding and more apoptosis cells.Omega group was significantly lighter than LPS group (P<0.01).2)Compared with control group, the lung indexes and pathological scores of ALI model groups were significantly higher (P<0.05).3) The levels of TNF-α, IL-1β and IL-6 in BALF of LPS group were significantely elevated than omega group (both P＜0.05). Conclusion 10%Omegaven can relieve inflammation by downregulating the concentrations of TNF-α, IL-1β and IL-6 and reduce lung injury.
关键词
ω-3PUFAs /
急性肺损伤 /
肿瘤坏死因子-α /
白介素1-β /
白介素6
Key words
ω-3PUFAs /
acute lung injury /
TNF-α /
IL-1β /
IL-6
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基金
浙江省医学重点扶植学科“临床营养学”(2007-F03)
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