目的 以常用的注意缺陷多动障碍(attention deficit hyperactivity disorder,ADHD)模型自发性高血压大鼠(spontaneously hypertensive rats,SHR)为观察对象,观察其与注意缺陷多动障碍临床特征在行为学上的相似性。方法 SHR、SD(Sprague Dawley rats,SD)、WKY(Wistar Kyoto rats,WKY)大鼠各10只,利用开场实验观察记录大鼠穿越的格子数、直立次数、理毛次数、粪便颗粒数。结果 反映大鼠活动能力的穿越格数及直立次数,SHR大鼠均明显较对照组WKY、SD大鼠增多,差异有高度统计学意义(P<0.01);而表现情绪紧张程度的理毛次数和排便粒数,SHR大鼠与对照组WKY、SD大鼠的差异无统计学意义(P>0.05)。结论 SHR大鼠的多动性明显高于SD、WKY大鼠,能很好的再现注意缺陷多动障碍的多动行为学特征,是比较理想的注意缺陷多动障碍的动物模型。
Abstract
Objective To observe the behavioral characteristics of animal models of spontaneously hypertensive rats (SHR) with attention deficit hyperactivity disorder (ADHD)on the behavior of the similarity. Methods 10 spontaneously hypertensive rats (SHR),Sprague Dawley rats (SD),Wistar Kyoto rats (WKY) rats in each.The panels the rats passed,the times of straighten and grooming,and times of dejecture were observed and recorded by using open-field environment test. Results The panels passed and the times of straightening SHR rats were significantly higher than those of the SD and WKY rats(P<0.01);the times of grooming and dejecture of SHR rats had no differences with the other groups(P>0.05). Conclusions SHR rats with significantly higher hyperactivity than SD WKY rats,could be a very good representation of hyperactivity behavior of characteristics,and be ideal animal models of ADHD.
关键词
注意缺陷多动障碍 /
自发性高血压大鼠 /
WKY /
SD /
开场实验
Key words
attention deficit hyperactivity disorder /
spontaneously hypertensive rats /
Wistar Kyoto rats /
Sprague Dawley rats /
the open field test
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参考文献
[1] Polanczyk G,deLima,Horta B,et al.The worldwide prevalence of ADHD:a systematic review and metaregression analysis[J].Am J Psychiatry,2007,164(6):942-948.
[2] Meneses A,Perez-Garcia G,Ponce-Lopez T,et al.Spontaneously hypertensive rat(SHR) as an animal model for ADHD:a short overview[J].Rev Neurosci,2011,22(3): 365-71.
[3] Roessner V,Sagvolden T,Dasbanerjee T,et al.Methylphenidate normalizes elevated dopamine transporter densities in an animal model of the attention-deficit/hyperactivity disorder combined type,but not to the same extent in one of the attention-deficit/hyperactivity disorder inattentive type[J].Neuroscience,2010,167(4):1183-1191.
[4] Yang PB,Swann AC,Dafny N.Psychostimulants given in adolescents modulate their effects in adulthood using the open field and the wheel-running assays[J].Brain Res Bull,2010,82(3-4):208-217.
[5] Chelaru MI,Yang PB,Dafny N.Sex differences in the behavioral response to methylphenidate in three adolescent rat strains (WKY,SHR,SD)[J].Behav Brain Res,2012,226(1):8-17.
[6] Langen B,Dost R.Comparison of SHR,WKY and Wistar rats in different behavioural animal models: effect of dopamine D1 and alpha2 agonists[J].Atten Defic Hyperact Disord,2011,3(1):1-12.
[7] Cao AH,Yu L,Wang YW,et al.Composition of long chain polyunsaturated fatty acids (LC-PUFAs) in different encephalic regions and its association with behavior in spontaneous hypertensive rat (SHR)[J].Brain Res,2013,152(8):49-57.
[8] Li Q,Lu G,Antonio GE,et al.The usefulness of the spontaneously hypertensive rat to model attention-deficit/hyperactivity disorder (ADHD) may be explained by the differential expression of dopamine-related genes in the brain[J].Neuro Chem Int,2007,50(6):848-857.
[9] Umehara M,Ago Y,Fujita K,et al.Effects of serotonin-norepinephrine reuptake inhibitors on locomotion and prefrontal monoamine release in spontaneously hypertensive rats[J].Eur J Pharmacol,2013;702(1-3):250-257.
[10] Sagvolden T,Johansen EB,Woien G,et al.The spontaneously hypertensive rat model of ADHD-The importance of selecting the appropriate reference strain[J].Neuropharmacology,2009,57(7-8):619-626.
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