目的 探讨母鼠妊娠前慢性不可预见性应激(chronic unpredictable stress,CUS)对1月龄子鼠学习记忆能力及大脑海马突触结合蛋白 I(synaptotagmin I,syt I)和突触结合蛋白 IV(syt IV)表达的影响。方法 SD系成年健康雌性大鼠随机分为正常对照组(8只)和CUS组(10只);采用11种刺激方法构建CUS模型;子鼠1月龄时行Morris水迷宫试验;应用Elisa法检测实验动物血清的皮质醇(cortisol,COR)和促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)水平;采用免疫组织化学法检测子鼠大脑海马syt I和syt IV的表达情况。 结果 1)应激结束时和分娩后,CUS组母鼠血清CRH和COR水平均高于正常对照组。2)水迷宫实验中,CUS组子鼠寻找平台潜伏期长于正常对照组子鼠,而穿越平台次数少于正常对照组子鼠,差异有统计学意义。3)1月龄CUS组子代CRH和COR水平均高于正常组子代。4)CUS组和正常对照组子鼠大脑海马syt I和syt IV蛋白表达的平均光密度(MOD)值,只在CA3层和DG层差异均有统计学意义。 结论 母鼠妊娠前CUS会使子鼠的学习记忆能力下降,并使大脑海马syt I和syt IV的表达减少。
Abstract
Objective To investigate the effect of pre-gestational stress on the spacial memory of the offspring rats as well as the expression of synaptotagmin I (syt I) and synaptotagmin IV (syt IV) in hippocampus when they were one month old. Methods Adult female SD rats were divided randomly into two groups:control group (n=8) and chronic unpredictable stress (CUS) group (n=12).The spacial memory of the offspring rats was tested in Morris water maze when they were one month old.Serum cortisol (COR) and corticotropin-releasing hormone (CRH) levels of maternal rats and offspring rats were determined by using a standard Elisa kit.The expression of syt I and syt IV in the hippocampus of the offspring rats were studied by immunohistochemistry. Results 1)After the stress procedure and post-delivery,maternal serum CRH and COR level in CUS group were higher than control group.2)Morris water maze test showed that the escape latency of offspring rats in CUS group were longer than in control group; Number of times that the rat crossed the position of offspring rats in CUS group were less than in control group.3)The CRH and COR level of offspring rats in CUS group were higher than in control group.4)There were statistical differences of the mean optical density (MOD) of syt I and syt IV at GA3,DG in the offspring rats between cus group and control group (All P<0.05). Conclusion Pre-gestational stress impaires the spacial memory of the offspring rats and reduces the expressions of syt I and syt IV in hippocampus of them.
关键词
慢性不可预见性应激 /
学习 /
皮质醇 /
促肾上腺皮质激素释放激素 /
突触结合蛋白I /
突触结合蛋白IV
Key words
chronic unpredictable stress /
learning /
cortisol /
corticotropin-releasing hormone /
synaptotagmin I /
synaptotagmin IV
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基金
国家自然科学基金资助项目(31140016);广东省科技计划项目(2010B031600282)
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