关键词: 维生素A, 肺, 形态, 视黄酸受体, 胎鼠

Abstract: 【Objective】 To observe the effects of marginal vitamin A deficiency during pregnancy on the lung' morphology of fetal rats. 【Methods】 Prenatal vitamin A normal(VAN)and marginal vitamin A deficiency(MVAD)of Sprague-Dawley rat models were employed.There were 6 mice in each group.On the gestational day 19,fetal rats were harvested by cesarean section.Then weighed their body,lung and liver.The fetal lung morphology were observed by light microscope after hematoxylin and eosin stain.The vitamin A concentrations of lung were detected by high-performance liquid chromatography(HPLC),and the levels of retinoic acid receptor(RAR)mRNAs were detected by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR),then the values of RAR mRNAs were compared to glyceraldehyde-3-phosphate dehydrogenase(GAPDH)mRNA. 【Results】 1)The weights of fetals'body,lung and liver in VAN group were higher than those of MVAD group(P<0.05).2)By light microscope(high and lower power lens),the fetal lung morphology were better developed in VAN group than MVAD group.In VAN group,alveolar-like structure were better-arranged,and alveolar septums were thinner.They were rich in blood capillary among the interstitial tissue.They were canalicular period;but MVAD group were gland period.3)Compared with the two groups,the vitamin A concentrations of lungs in VAN group were higher than those of MVAD group(P<0.05).4)The levels of RAR and mRNA in VAN group were lower than those of MVAD group(P<0.05). 【Conclusions】 The weights of fetals' body,lung and liver,the fetal lung morphology,the vitamin A concentrations of lungs and the levels of RAR and mRNA are all influenced by vitamin A level during pregnancy.The fetal lung whose mother are vitamin A deficiency during pregnancy are worse developed.

Key words: vitamin A, lung, morphology, retinoic acid receptor, fetal rats