【目的】 观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体(sTRAILR1和sTRAILR4)在毛细支气管炎和急性上呼吸道感染患儿中的表达,探讨它们在呼吸道感染炎症机制中的作用。 【方法】 ELISA 法检测毛细支气管炎和急性上呼吸道感染患儿及健康对照组儿童血清sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度。 【结果】 毛细支气管炎组和急性上呼吸道感染组患者血清中sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度分别为(85±38)、(71±19)、(167±97);(78±28)、(61±15)、(139±72) pg/mL均显著高于对照组(55±19)、(45±14)、(56±38) pg/mL(P均<0.01);毛细支气管炎组sTRAILR1的蛋白浓度显著高于急性上呼吸道感染组(P<0.05),两组sTRAIL和sTRAILR4的蛋白浓度相比差异无统计学意义(P均>0.05)。sTRAILR1分别与sTRAIL、sTRAILR4的蛋白浓度呈显著正相关(n=102,r分别为0.236和0.409,P均<0.01)。 【结论】 毛细支气管炎和急性上呼吸道感染患儿血清中可溶性TRAIL及其受体TRAILR1和TRAILR4的表达增加,它们可能参与了呼吸道感染的炎症过程,TRAIL可能主要通过TRAILR1起作用,它们的表达水平增高可能被视为病情活动的指标之一。
Abstract
【Objective】 To investigate the potential roles of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its soluble receptors (sTRAILR1 and sTRAILR4) in the pathogenesis of respiratory tract infections,the concentrations of sTRAIL,sTRAILR1 and sTRAILR4 proteins were determined. 【Methods】 Concentrations of sTRAIL and sTRAILR1 and sTRAILR4 protein were detected by ELISA,in patients with bronchiolitis and acute upper respiratory infection and individuals(named bronchiolitis group,AURI group,control group). 【Results】 The concentrations of sTRAIL,sTRAILR1 and sTRAILR4 protein were significantly higher in bronchiolitis group (85±38),(71±19),(167±97) pg/mL,respectively and in AURI group(78±28),(61±15),(139±72) pg/mL than those in control group(55±19),(45±14),(56±38) pg/mL,respectively(P all<0.01).Data also showed that the concentration of sTRAILR1 protein was markedly higher in bronchiolitis group than that in AURI group(P all<0.05).While no significance were observed of sTRAIL and sTRAILR4 expressions between the patients with bronchiolitis group and in AURI group(P>0.05).Furthermore,levels of sTRAILR1 was highly correlated to sTRAIL and sTRAILR4(n=102,r=0.236 and 0.409 respectively,all P<0.01). 【Conclusion】 Levels of sTRAIL,sTRAILR1 and sTRAILR4 were elevated in bronchiolitis and AURI which could be applied as an effective targets of patient's condition judgment.Our study indicated TRAIL and its receptors may involved in the pathogenesis of bronchiolitis and AURI inflammation.
关键词
毛细支气管炎 /
急性上呼吸道感染 /
肿瘤坏死因子相关凋亡诱导配体 /
受体 /
酶联免疫吸附试验
Key words
enzyme linked immunosorbent assay /
bronchiolitis /
acute upper respiratory infection /
tumor necrosis factor related apoptosis inducing ligand /
receptor
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参考文献
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基金
浙江省医药卫生科学研究基金计划(2009B169);温岭市科技局基金资助项目(2010-1-73)
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