毛细支气管炎和急性上呼吸道感染患儿血清可溶性TRAIL及其受体TRAILR1、TRAILR4的表达

中国儿童保健杂志 ›› 2012, Vol. 20 ›› Issue (1): 75-77.

毛细支气管炎和急性上呼吸道感染患儿血清可溶性TRAIL及其受体TRAILR1、TRAILR4的表达

江德富¹,陶荣辉¹,童夏生²,陈素萍¹,王晔华¹,王恩智¹

1 温岭市第二人民医院,浙江台州 317500;
2 台州市中西医结合医院,浙江台州 317523

收稿日期:2011-08-30 发布日期:2012-12-06 出版日期:2012-01-06
作者简介:江德富(1965-),男,浙江人,副主任医师,学士学位,研究方向为儿科呼吸系统疾病
基金资助:
浙江省医药卫生科学研究基金计划(2009B169);温岭市科技局基金资助项目(2010-1-73)

Expressions of serum soluble tumor necrosis factor related apoptosis inducing ligand and its receptor 1 and 4 in patients with bronchiolitis and acute upper respiratory infection

JIANG De-fu¹,TAO Rong-hui¹,TONG Xia-sheng¹,CHEN Su-ping¹,WANG Ye-hua¹,WANG En-zhi¹

1 The Second People's Hospital of Wenling,Taizhou,Zhejiang 317500,China; 2 Taizhou Integrated Western and Traditional Chinese Medicine Hospital,Taizhou,Zhejiang 317523,China

Received:2011-08-30 Online:2012-01-06 Published:2012-12-06

摘要/Abstract

摘要： 【目的】观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体(sTRAILR1和sTRAILR4)在毛细支气管炎和急性上呼吸道感染患儿中的表达,探讨它们在呼吸道感染炎症机制中的作用。【方法】 ELISA 法检测毛细支气管炎和急性上呼吸道感染患儿及健康对照组儿童血清sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度。【结果】毛细支气管炎组和急性上呼吸道感染组患者血清中sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度分别为(85±38)、(71±19)、(167±97);(78±28)、(61±15)、(139±72) pg/mL均显著高于对照组(55±19)、(45±14)、(56±38) pg/mL(P均<0.01);毛细支气管炎组sTRAILR1的蛋白浓度显著高于急性上呼吸道感染组(P<0.05),两组sTRAIL和sTRAILR4的蛋白浓度相比差异无统计学意义(P均>0.05)。sTRAILR1分别与sTRAIL、sTRAILR4的蛋白浓度呈显著正相关(n=102,r分别为0.236和0.409,P均<0.01)。【结论】毛细支气管炎和急性上呼吸道感染患儿血清中可溶性TRAIL及其受体TRAILR1和TRAILR4的表达增加,它们可能参与了呼吸道感染的炎症过程,TRAIL可能主要通过TRAILR1起作用,它们的表达水平增高可能被视为病情活动的指标之一。

关键词: 毛细支气管炎, 急性上呼吸道感染, 肿瘤坏死因子相关凋亡诱导配体, 受体, 酶联免疫吸附试验

Abstract: 【Objective】 To investigate the potential roles of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its soluble receptors (sTRAILR1 and sTRAILR4) in the pathogenesis of respiratory tract infections,the concentrations of sTRAIL,sTRAILR1 and sTRAILR4 proteins were determined. 【Methods】 Concentrations of sTRAIL and sTRAILR1 and sTRAILR4 protein were detected by ELISA,in patients with bronchiolitis and acute upper respiratory infection and individuals(named bronchiolitis group,AURI group,control group). 【Results】 The concentrations of sTRAIL,sTRAILR1 and sTRAILR4 protein were significantly higher in bronchiolitis group (85±38),(71±19),(167±97) pg/mL,respectively and in AURI group(78±28),(61±15),(139±72) pg/mL than those in control group(55±19),(45±14),(56±38) pg/mL,respectively(P all<0.01).Data also showed that the concentration of sTRAILR1 protein was markedly higher in bronchiolitis group than that in AURI group(P all<0.05).While no significance were observed of sTRAIL and sTRAILR4 expressions between the patients with bronchiolitis group and in AURI group(P>0.05).Furthermore,levels of sTRAILR1 was highly correlated to sTRAIL and sTRAILR4(n=102,r=0.236 and 0.409 respectively,all P<0.01). 【Conclusion】 Levels of sTRAIL,sTRAILR1 and sTRAILR4 were elevated in bronchiolitis and AURI which could be applied as an effective targets of patient's condition judgment.Our study indicated TRAIL and its receptors may involved in the pathogenesis of bronchiolitis and AURI inflammation.

Key words: enzyme linked immunosorbent assay, bronchiolitis, acute upper respiratory infection, tumor necrosis factor related apoptosis inducing ligand, receptor

中图分类号:

R725.6

江德富,陶荣辉,童夏生,陈素萍,王晔华,王恩智. 毛细支气管炎和急性上呼吸道感染患儿血清可溶性TRAIL及其受体TRAILR1、TRAILR4的表达[J]. 中国儿童保健杂志, 2012, 20(1): 75-77.

JIANG De-fu,TAO Rong-hui,TONG Xia-sheng,CHEN Su-ping,WANG Ye-hua,WANG En-zhi. Expressions of serum soluble tumor necrosis factor related apoptosis inducing ligand and its receptor 1 and 4 in patients with bronchiolitis and acute upper respiratory infection[J]. journal1, 2012, 20(1): 75-77.

参考文献

[1] Cheong HJ,Lee KS,Woo IS,et al.Up-regulation of the DR5 Expression by Proteasome Inhibitor MG132 Augments TRAIL-Induced Apoptosis in Soft Tissue Sarcoma Cell Lines[J].Cancer Res Treat,2011,43(2):124-130.
[2] Guo L,Fan L,Ren J,et al.A novel combination of TRAIL and doxorubicin enhances antitumor effect based on passive tumor-targeting of liposomes[J].Nanotechnology,2011,22(26):265105-265107.
[3] Cziupka K,Busemann A,Partecke LI,et al.Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) improves the innate immune response and enhances survival in murine polymicrobial sepsis[J].Crit Care Med,2010,38(11):2169-2174.
[4] Stegmann KA,Bjrkstrm NK,Veber H,et al.Interferon-alpha-induced TRAIL on natural killer cells is associated with control of hepatitis C virus infection[J].Gastroenterology,2010,138(5):1885-1897.
[5] 胡亚美,江载芳.实用儿科学[M].7版.北京:人民卫生出版社, 2002:1169,1180.
[6] Thanaketpaisarn O,Waiwut P,Sakurai H,et al.Artesunate enhances TRAIL-induced apoptosis in human cervical carcinoma cells through inhibition of the NF-κB and PI3K/Akt signaling pathways[J].Int J Oncol,2011,39(1):279-285.
[7] 高志华,陈主初.TRAIL及其受体介导的信号转导通路[J].生命的化学,2001,21(3):212-214.
[8] Warke RV,Martin KJ,Giaya K,et al.TRAIL is a novel antiviral protein against dengue virus[J].J Virol,2008,82(1):555-564.
[9] Stegmann KA,Bjrkstrm NK,Veber H,et al.Interferon-alpha-induced TRAIL on natural killer cells is associated with control of hepatitis C virus infection[J].Gastroenterology,2010,138(5):1885-1897.
[10] Duttagupta PA,Boesteanu AC,Katsikis PD.Costimulation signals for memory CD8⁺ T cells during viral infections[J].Crit Rev Immunol,2009,29(6):469-486.
[11] Kotelkin A,Prikhod'ko EA,Cohen JI,et al.Respiratory syncytial virus infection sensitizes cells to apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand[J].J Virol,2003,77(17):9156-9172.
[12] Herold S,Steinmueller M,von Wulffen W,et al.Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand[J].J Exp Med,2008,205(13):3065-3077.
[13] Brincks EL,Kucaba TA,Legge KL,et al.Influenza-induced expression of functional tumor necrosis factor-related apoptosis-inducing ligand on human peripheral blood mononuclear cells[J].Hum Immunol,2008,69(10):634-646.
[14] Robertson NM,Rosemiller M,Lindemeyer RG,et al.TRAIL in the airways[J].Vitam Horm,2004,67:149-167.

[1]	李帆, 杨可欣, 张旭童, 陈凌波, 刘春豪, 徐鹏程, 胡浩, 孙晓明. 奶茶摄入诱导幼龄小鼠糖脂代谢紊乱的研究[J]. 中国儿童保健杂志, 2024, 32(9): 974-979.
[2]	邱佳琪 , 钟霞, 邹时朴. 多巴胺及其受体在儿童抽动障碍中的作用[J]. 中国儿童保健杂志, 2024, 32(10): 1097-1100.
[3]	熊萍, 朱红敏, 黄馨, 吴静, 林晶, 钟严艳. 离子型谷氨酸受体基因遗传变异与儿童注意缺陷多动障碍关联性研究[J]. 中国儿童保健杂志, 2023, 31(9): 945-950.
[4]	李嘉, 张延赤. 维生素D受体基因多态性及维生素D水平与神经发育障碍关系的研究进展[J]. 中国儿童保健杂志, 2023, 31(5): 516-520.
[5]	王旭, 王书影, 黎历, 邹映雪. 毛细支气管炎后复发喘息危险因素的Meta分析[J]. 中国儿童保健杂志, 2023, 31(2): 190-194.
[6]	崔一帆, 肖绪武. 维生素D及其受体基因多态性与儿童疾病的相关性[J]. 中国儿童保健杂志, 2023, 31(10): 1111-1115.
[7]	田渤, 赵凯红, 赵怡然, 付洪涛. 外周血单核细胞髓样分化因子88与Toll样受体4在早产儿窒息后多器官功能障碍综合征中的表达及其意义[J]. 中国儿童保健杂志, 2022, 30(4): 454-457.
[8]	陶凤姣, 温航卫, 刘作姣. 毛细支气管炎患儿血清高迁移率族蛋白B₁、可溶性清道夫受体163水平与呼出气一氧化氮浓度及病情严重程度的关系[J]. 中国儿童保健杂志, 2022, 30(3): 335-338.
[9]	陈国萍, 肇颖新. Toll样受体4对新生儿坏死性小肠结肠炎作用的研究现状[J]. 中国儿童保健杂志, 2022, 30(2): 167-171.
[10]	赵铭, 秦苗, 孙梦雅, 姜红, 李向红, 姜健, 刘燕. NLRP3及相关炎性因子在新生大鼠坏死性小肠结肠炎中的表达研究[J]. 中国儿童保健杂志, 2021, 29(8): 852-856.
[11]	董明瑛, 陶炳铜. 白三烯C4合成酶基因多态性与呼吸道合胞病毒毛细支气管炎易感性及疗效的相关性[J]. 中国儿童保健杂志, 2021, 29(5): 545-549.
[12]	秦莹, 仰曙芬. 维生素D与脑发育相关研究进展[J]. 中国儿童保健杂志, 2021, 29(4): 393-396.
[13]	吴少辉, 黄晓冬, 徐智航. TLRs信号通路和炎症因子的表达与反复呼吸道感染的关系分析[J]. 中国儿童保健杂志, 2021, 29(2): 205-208.
[14]	唐静, 李涛, 孟林. 血清Toll样受体4对重症哮喘患儿治疗后1年内再发风险的预测价值[J]. 中国儿童保健杂志, 2021, 29(12): 1372-1375.
[15]	罗春绸, 蔡惠贞, 张宝忠, 林秀梅, 杨惠恋, 陈贤桂, 林英. 入托对儿童急性上呼吸道感染发病及咽部病原体变化的影响[J]. 中国儿童保健杂志, 2020, 28(8): 943-946.