【目的】 分析新生儿接种卡介苗(bacillus calmette-guerin,BCG)后细胞免疫功能变化。 【方法】 收集150例BCG接种后结核菌素纯蛋白衍生物(purified protein deriva-tive,PPD)阳性婴儿(接种成功组)和50例BCG接种后PPD阴性婴儿(接种失败组)的外周静脉血,应用ELISA方法测定血清细胞因子IFN-γ和IL-10的含量,评价细胞免疫功能。 【结果】 接种成功组和接种失败组血清细胞因子IFN-γ含量分别为(49.78±12.20)和(38.08±7.68)pg/mL,差异具有统计学意义(P=0.001);血清IL-10含量分别为(3.60±2.00)和(5.71±2.71)pg/mL,差异具有统计学意义(P<0.01)。 【结论】 BCG接种成功(PPD阳性)婴儿的细胞免疫功能增强。
Abstract
【Objective】 To analyze the change in cell-mediated function in infants vaccinated with BCG. 【Methods】 Peripheral blood specimen from 150 infants with positive PPD test (Group successfully Vaccination,SV) and 50 ones with negative PPD test (Group failure vaccination,FV) were collected.The cytokine IFN-γ and IL-10 concentrations were measured by ELISA to evaluate cell-mediated immunity function after BCG vaccination. 【Results】 Serum IFN-γ concentrations in Groups SV and FV were (49.78±12.20)and (38.08±7.68)pg/mL,respectively(P=0.001).Serum IL-10 contents in Group SV and FV were (3.60±2.00) and (5.71±2.71)pg/mL,respectively (P<0.01). 【Conclusion】 The infants vaccinated successfully (positive PPD test) had an increasing cell-mediated immunity function.
关键词
卡介苗 /
细胞免疫 /
细胞因子
Key words
bacillus calmette-guerin /
cell-mediated immunity /
cytokine
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] World Health Organization.Word health report 2000.Health systems improving performance[R].Geneva:WHO,2000.
[2] 杨连清.影响新生儿卡介苗接种阳转率的因素分析[J].现代预防医学,2008,35(13):2437-2438.
[3] 吴守芝,宋俊峰.结核分支杆菌致病机制与免疫[J].中华结核和呼吸杂志,2003,26(2):101-103.
[4] 赵平.结核分支杆菌细胞免疫学研究进展[J].中国人兽共患病杂志,2006,22(2):182-185.
[5] 焦新安,Richard LM,Pierre G,et al.重组卡介苗诱导T细胞免疫应答的分子、细胞机理研究[D].扬州大学,2000.
[6] 周晖,范艳莹,吴长有.卡介苗( BCG)通过诱导IL-12产生和受体表达促进人NK细胞功能[J].细胞与分子免疫学杂志,2009,25(11):976-983.
[7] Vander Bruggen P,Traversari C.A gene encoding an antigen recog.nized by cytolytic T lymphocytes on a human melanoma[J].J Immunol,2007,178(5):2617-2621.
[8] 李晓秋,张建华.卡介苗在支气管哮喘防治中的研究进展[J].国际儿科杂志,2006,33(5):330-332.
[9] 姜蔚.卡介苗接种后结核菌素蛋白衍生物试验与细胞免疫的关系[J].海军医学杂志,2005,26(3):222-224.
PDF(422 KB)
