支气管哮喘患儿血清肿瘤坏死因子α及其可溶性受体1和2的检测及临床意义
- 童夏生1,李晨虹1,方丽2,王恩智1,叶辉3
作者信息
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Clinical significance of serum tumor necrosis factor and it's soluble receptor 1 and 2 in patients with bronchial asthma
- TONG Xia-sheng1, LI Chen-hong1, FANG Li2, WANG En-zhi1, YE Hui3
Author information
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文章历史
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摘要
【目的】 观察肿瘤坏死因子-α(TNF-α)及其可能性受体(sTNF-R1和sTNF-R2)在支气管哮喘患者中的表达,探讨它们在支气管哮喘炎症机制中的作用。 【方法】 采用ELISA法检测哮喘急性发作期和哮喘缓解期患儿及健康对照组儿童血清TNF-α、sTNF-R1和sTNF-R2的蛋白浓度。 【结果】 哮喘急性发作期组血清TNF-α、sTNF-R1 和sTNF-R2的蛋白浓度[分别为(3.19±1.68)、(4.69±2.30)、(14.32±6.19)] ng/mL显著高于对照组[(1.96±0.86)、(2.07±0.86)、(4.75±1.68)] ng/mL (P均＜0.01);哮喘缓解期组sTNF-R1 和sTNF-R2的蛋白浓度[分别为(2.59±1.21)、(11.57±4.78)] ng/mL显著低于哮喘急性发作期组(分别为P＜0.01和＜0.05),而TNF-α的蛋白浓度(2.75±1.31) ng/mL与哮喘急性发作期组相比差异无统计学意义(P>0.05);哮喘缓解期组TNF-α和sTNF-R2的蛋白浓度显著高于对照组(P均＜0.01),而sTNF-R1的蛋白浓度与对照组相比差异无统计学意义。sTNF-R1与sTNF-R2的表达水平呈显著正相关(n=99,r =0.239,P＜0.05),但sTNF-R1和sTNF-R2的表达水平分别与TNF-α之间无显著相关性(P>0.05)。 【结论】 TNF-α、sTNFR1和sTNFR2可能参与了哮喘的炎症发病过程,哮喘患者血清TNF-α、sTNF-R1和sTNF-R2水平增高可能被作为病情活动的指标之一。
Abstract
【Objective】 To investigate the potential roles of tumor necrosis factor-α(TNF-α) and it's solube receptors(sTNF-R1 and sTNF-R2) in the pathogenesis of asthmatic inflammation, the concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were determined. 【Methods】 All the patients were obtained including 46 cases of patients with bronchial asthma exacerbation, 31 cases of patients with bronchial asthma paracmasia, and were compared to 22 cases of individuals. Concentrations of TNF-α proteins was detected by enzyme linked immunosorbent assay methods(ELISA). And the concentrations of sTNF-R1 and sTNF-R2 proteins were also determined by ELISA. 【Results】 The concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were significantly higher in asthma exacerbation group[(3.19±1.68),(4.69±2.30),(14.32±6.19) ng/mL respectively] than those in control group [(1.96±0.86)、(2.07±0.86)、(4.75±1.68) ng/mL respectively](all P<0.01). Data also showed that the concentrations of sTNF-R1 and sTNF-R2 proteins were markedly lower in bronchial asthma paracmasia group [(2.59±1.21)、(11.57±4.78) ng/mL respectively] than those in asthma exacerbation group(P＜0.01 or ＜0.05, respectively). While no significance were observed of TNF-αexpressions between the patients with asthma paracmasia group(2.75±1.31) ng/mL and in acute phase(P>0.05). When compared to control group, the concentrations of TNF-α and sTNF-R2 proteins in asthma paracmasia group were significantly higher, but there were no significance of sTNF-R1 proteins between them. Furthermore, levels of sTNF-R1 was highly correlated to sTNF-R2(n=99,r=0.239,P＜0.05), but there were no strong association neither between levels of sTNF-R1 and TNF-α, nor between levels of sTNF-R2 and TNF-α(all P>0.05). 【Conclusions】 Levels of TNF-α, sTNF-R1 and sTNF-R2 are elevated in bronchial asthma which could be applied as an effective targets of patient's condition judgment. Our study indicated TNF-α, sTNF-R1 and sTNF-R2 expressions may involved in the pathogenesis of bronchial asthma inflammation.
关键词
Key words
bronchial asthma / tumor necrosis factor / receptor / enzyme linked immunosorbent assay
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参考文献
[1] 中华医学会儿科学分会呼吸组,《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南[J].中华儿科杂志,2008,46(10):745-753.
[2] Puthothu B, Bierbaum S, Kopp MV, et al. Association of TNF-alpha with severe respiratory syncytial virus infection and bronchial asthma[J]. Pediatr Allergy Immunol, 2009,20(2):157-163.
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[4] 宋一萍,尤胜义.肿瘤坏死因子与儿童哮喘[J].中国全科医学,2004,7(9):1365-1366.
[5] Parthenakis FI, Patrianakos A, Prassopoulos V, et al. Relation of cardiac sympathetic innervation to proinflammatory cytokine levels in patients with heart failure secondary to idiopathic dilated cardiomyopathy[J]. Am J Cardiol,2003,91(10):1190-1194.
[6] 龚柳阳,王雪芬.肿瘤坏死因子-α与哮喘[J].国际呼吸杂志,2007,27(20):1545-1547.
[7] Amrani Y, Chen H, Panettieri RA. Activation of tumor necrosis factor receptor 1 in airway smooth muscle: a potential pathway that modulates bronchial hyper-responsiveness in asthma?[J]. Respir Res,2000,1(1):49-53.
[8] Hughes JM, Rimmer SJ, Salome CM, et al. Eosinophilia, interleukin-5, and tumour necrosis factor-alpha in asthmatic children[J]. Allergy,2001,56(5):412-418.
[9] Sikora JP, Kuzański W, Andrzejewska E. Soluble cytokine receptors sTNFR I and sTNFR II, receptor antagonist IL-1ra, and anti-inflammatory cytokines IL-10 and IL-13 in the pathogenesis of systemic inflammatory response syndrome in the course of burns in children[J]. Med Sci Monit,2009,15(1):26-31.
[10] Lombardi V, Singh AK, Akbari O. The role of costimulatory molecules in allergic disease and asthma[J]. Int Arch Allergy Immunol,2010,151(3):179-189.
[11] Fang L, Adkins B, Deyev V, et al. Essential role of TNF receptor superfamily 25(TNFRSF25) in the development of allergic lung inflammation[J]. J Exp Med,2008,205(5):1037-1048.
[12] Sumitomo M, Suda S, Shindo K, et al. Serum levels of tumor necrosis factor alpha and soluble tumor necrosis factor-receptor I in asthmatic patients and patients with chronic respiratory tract infection[J]. Arerugi,1997,46(11):1136-1147.
[13] 张坚松,王悦,向阳,等.哮喘患儿可溶性肿瘤坏死因子受体Ⅰ的表达[J].湖南师范大学学报:医学版,2005,2(2):4-6.
[2] Puthothu B, Bierbaum S, Kopp MV, et al. Association of TNF-alpha with severe respiratory syncytial virus infection and bronchial asthma[J]. Pediatr Allergy Immunol, 2009,20(2):157-163.
[3] Chung JY, Han TH, Kim JS, et al. Th1 and Th2 cytokine levels in nasopharyngeal aspirates from children with human bocavirus bronchiolitis[J]. J Clin Virol,2008,43(2):223-235.
[4] 宋一萍,尤胜义.肿瘤坏死因子与儿童哮喘[J].中国全科医学,2004,7(9):1365-1366.
[5] Parthenakis FI, Patrianakos A, Prassopoulos V, et al. Relation of cardiac sympathetic innervation to proinflammatory cytokine levels in patients with heart failure secondary to idiopathic dilated cardiomyopathy[J]. Am J Cardiol,2003,91(10):1190-1194.
[6] 龚柳阳,王雪芬.肿瘤坏死因子-α与哮喘[J].国际呼吸杂志,2007,27(20):1545-1547.
[7] Amrani Y, Chen H, Panettieri RA. Activation of tumor necrosis factor receptor 1 in airway smooth muscle: a potential pathway that modulates bronchial hyper-responsiveness in asthma?[J]. Respir Res,2000,1(1):49-53.
[8] Hughes JM, Rimmer SJ, Salome CM, et al. Eosinophilia, interleukin-5, and tumour necrosis factor-alpha in asthmatic children[J]. Allergy,2001,56(5):412-418.
[9] Sikora JP, Kuzański W, Andrzejewska E. Soluble cytokine receptors sTNFR I and sTNFR II, receptor antagonist IL-1ra, and anti-inflammatory cytokines IL-10 and IL-13 in the pathogenesis of systemic inflammatory response syndrome in the course of burns in children[J]. Med Sci Monit,2009,15(1):26-31.
[10] Lombardi V, Singh AK, Akbari O. The role of costimulatory molecules in allergic disease and asthma[J]. Int Arch Allergy Immunol,2010,151(3):179-189.
[11] Fang L, Adkins B, Deyev V, et al. Essential role of TNF receptor superfamily 25(TNFRSF25) in the development of allergic lung inflammation[J]. J Exp Med,2008,205(5):1037-1048.
[12] Sumitomo M, Suda S, Shindo K, et al. Serum levels of tumor necrosis factor alpha and soluble tumor necrosis factor-receptor I in asthmatic patients and patients with chronic respiratory tract infection[J]. Arerugi,1997,46(11):1136-1147.
[13] 张坚松,王悦,向阳,等.哮喘患儿可溶性肿瘤坏死因子受体Ⅰ的表达[J].湖南师范大学学报:医学版,2005,2(2):4-6.
基金
浙江省医药卫生科学研究基金计划(2009B169);温岭市科技局基金资助项目(2008-63)
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