【目的】 通过基于1H-NMR的代谢组学方法检测早产儿并发呼吸窘迫综合征(neonatal respiratory distress syndrome, NRDS)的代谢产物,探讨代谢组学在早产儿疾病中的应用及寻找早产儿NRDS的早期生物标志物(群)。 【方法】 分为早产儿NRDS组和早产儿肺炎组,每组各20例;2组在入院年龄、胎龄、出生体重等方面具有可比性。所有病例于入院半小时内抽血、收集血浆,用1H-NMR方法检测血浆中的小分子物质和脂类代谢物。 【结果】 1)小分子物质分析:NRDS组与肺炎组相比,血浆中乳酸、某种脂质及乙醇增加,而葡萄糖、肌酸、谷氨酰胺、O-乙酰基(OAc)、N-乙酰基(NAc)、丙氨酸、缬氨酸、异亮氨酸降低;2)脂质分析:NRDS组与肺炎组相比,血浆中带HC=CH基团脂质、NAc、磷脂酰胆碱(PtdCho)、低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)降低。 【结论】 1)NRDS早产儿存在乳酸的堆积、主要供能物质葡萄糖及重要氨基酸等的减少;2)NRDS早产儿存在脂质代谢紊乱;3)代谢组学方法在早产儿疾病中有较大的应用前景。
Abstract
【Objective】 To detect the metabolite profiling and find the early biomarker or biomarkers in preterm infants with neonatal respiratory distress syndrome(NRDS) by a metabonomics technique based on 1H-NMR. 【Methods】 The preterm infants divided into 2 groups as NRDS group (NRDS) and pneumonia group(pneumonia) with 20 cases respectively. There was no difference in the age, gestational age and birth weight etc. Blood samples were drewed within 30 minutes after admission and the plasma were collected after centrifugalization. The plasma low-molecular mectabolites and lipid were detected. 【Results】 1)The concentrations of lactate, some lipid and alcohol were higher, the concentration of glucose, creatine, glutamine, OAc, NAc, alanine, valine, isoleucine were lower in NRDS group comparing to pneumonia group .2)The concentrations of HC=CH lipid, NAc, PtdCho, LDL and VLDL were lower in NRDS comparing to pneumonia. 【Conclusions】 Preterm infants with NRDS has lactate accumulation and lack the main energy substances like glucose and important amino acids with a lipid metabolic disturbance as while. Metabonomics has a great perspective in preterm infants diseases.
关键词
早产儿 /
新生儿呼吸窘迫综合症 /
代谢组学 /
主成分分析 /
偏最小二乘法-判别分析
Key words
preterm infants /
neonatal respiratory distress syndrome /
metabonomics /
principal component analysis /
partial-leasts-quares-discriminant
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参考文献
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基金
全国博士后基金资助项目(20080431407)
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