关键词: 早产儿, 新生儿呼吸窘迫综合症, 代谢组学, 主成分分析, 偏最小二乘法-判别分析

Abstract: 【Objective】 To detect the metabolite profiling and find the early biomarker or biomarkers in preterm infants with neonatal respiratory distress syndrome(NRDS) by a metabonomics technique based on ¹H-NMR. 【Methods】 The preterm infants divided into 2 groups as NRDS group (NRDS) and pneumonia group(pneumonia) with 20 cases respectively. There was no difference in the age, gestational age and birth weight etc. Blood samples were drewed within 30 minutes after admission and the plasma were collected after centrifugalization. The plasma low-molecular mectabolites and lipid were detected. 【Results】 1)The concentrations of lactate, some lipid and alcohol were higher, the concentration of glucose, creatine, glutamine, OAc, NAc, alanine, valine, isoleucine were lower in NRDS group comparing to pneumonia group .2)The concentrations of HC=CH lipid, NAc, PtdCho, LDL and VLDL were lower in NRDS comparing to pneumonia. 【Conclusions】 Preterm infants with NRDS has lactate accumulation and lack the main energy substances like glucose and important amino acids with a lipid metabolic disturbance as while. Metabonomics has a great perspective in preterm infants diseases.

Key words: preterm infants, neonatal respiratory distress syndrome, metabonomics, principal component analysis, partial-leasts-quares-discriminant