【目的】 观察哮喘大鼠肺组织病理改变和TRAF2表达的水平,探讨布地奈德治疗哮喘的可能作用机制。 【方法】 采用大鼠哮喘模型,随机分成哮喘组、对照组和布地奈德组,HE染色观察肺组织炎性改变,免疫组织化学法检测肺组织TRAF2的表达。 【结果】 哮喘组大鼠的毛发、体重、活动度等一般情况改变和肺组织炎性改变较对照组有显著变化,布地奈德能明显减轻上述改变。哮喘组(0.317±0.041 OD值)支气管壁TRAF2的光密度值显著高于对照组(0.220±0.057 OD值)(P＜0.01);布地奈德组支气管壁(0.236±0.033 OD值)TRAF2的光密度值显著低于哮喘组(P＜0.01),与对照组相比差异无统计学意义(P>0.05),但肺组织中其表达水平却显著高于哮喘组。 【结论】 哮喘大鼠TRAF2的表达水平增强,它可能参与了哮喘的气道炎症过程;布地奈德能减轻气道炎症,其机制可能是部分通过TRAF2途经。
Abstract
【Objective】 To investigate the potential roles of budesonide in asthma treatment, the pathological changes of asthmatic lung tissue and expressions of tumor necrosis factor receptor associated factor 2(TRAF2) were performed in rat asthma model. 【Methods】 The rats of asthma model were randomly divided into three groups on average, including asthma group, control group and budesonide treated group. The changes of lung inflammation were observed by hematoxylin eosin staining.The expressions of TRAF2 proteins were detected by immunohistochemical method either. 【Results】 There were significant differences between in asthma group and in control group about changes of lung inflammation and generral conditions of hairs, weight, activity, and so on . while they were significantly decreased by budesonide.Moreover, the expressions of TRAF2 proteins in asthma group (0.317±0.041 optical density)at bronchial wall were significantly higher than those in control group(0.220±0.057 optical density)(P＜0.01), and the expressions were dramaticly decreased in budesonide treated group(0.236±0.033 optical density) when compared to asthma group(P＜0.01). But there was no significant difference of TRAF2 protein expressions between in budesonide treated group and control group( P>0.05). 【Conclusions】 Levels of TRAF2 are increased in asthmatic rat, it may be as a potential role in pathogenesis of asthma inflammation. The function of budesonide on regulating TRAF2 levels may be partly through TRAF2 signal transduction pathway.
关键词
哮喘 /
肿瘤坏死因子受体相关因子 /
免疫组化 /
大鼠
Key words
asthma /
tumor necrosis factor receptor associated factor /
immunohistochemisty /
rat
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参考文献
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基金
浙江省卫生厅基金资助项目(2007-B238);温岭市科技局基金资助项目(2009-2-55)
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