目的 探究奶茶摄入对幼龄小鼠糖脂代谢的影响,为幼龄个体代谢紊乱的防治研究提供参考。方法 20只雄性ICR小鼠随机分为对照组与奶茶组,每组各10只。对照组小鼠正常饮水,奶茶组小鼠每天自由摄取奶茶和水各12h。2周后检测小鼠血糖、血脂变化,并检测不同组织中过氧化物酶体增殖物激活受体γ共激活因子1-α(PGC-1α)的蛋白水平。结果 与对照组相比,奶茶组小鼠体重在第2周出现了明显上升(t=2.402,P＜0.05),空腹血糖水平增高但差异无统计学意义(P＞0.05),糖耐量明显受损(P＜0.05);总胆固醇(T-CHO)和低密度脂蛋白胆固醇(LDL-C)显著升高(t=3.253、5.490,P＜0.05);相关性分析显示体重与血糖之间呈显著正相关(R2=0.71,P＜0.05);奶茶组与对照组相比,肝脏、骨骼肌和白色脂肪组织中PGC-1α的表达均被明显抑制(P＜0.05)。结论 奶茶摄入可诱导幼鼠糖脂代谢紊乱,可能与肝脏、骨骼肌和白色脂肪组织中PGC-1α的表达抑制有关。
Abstract
Objective To investigate the effect of milk tea consumption on glucose and lipid metabolism in young mice, so as to provide reference for the prevention and treatment of metabolic disorders in young individuals. Methods Twenty male ICR mice were randomly divided into the control group and milk tea group, with 10 mice in each group. Mice in the control group drank water normally, while mice in the milk tea group were given both water and milk tea for 12 hours each day. After 2 weeks, the blood glucose and lipids of mice were detected. The protein levels of peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α) in different tissues were detected. Results Compared with the control group, the body weight of mice in the milk tea group increased significantly at the end of the second week(t=2.402,P<0.05), the fasting blood glucose level increased but there was no significant difference(P>0.05), the glucose tolerance was significantly impaired(P<0.05), and total cholesterol(T-CHO) and low-density lipoprotein cholesterol(LDL-C) levels significantly increased(t=3.253,5.490,P<0.05). Correlation analysis showed that there was a significant positive correlation between body weight and blood glucose(R2=0.71,P<0.05). The expression levels of PGC-1α in liver, skeletal muscle and white adipose tissues of milk tea group were obviously inhibited(P<0.05). Conclusion The consumption of milk tea can induce glucose and lipid metabolism dysfunction in young mice, which may be related to the inhibition of PGC-1α expression in liver, skeletal muscle and white adipose tissues.
关键词
奶茶 /
葡萄糖代谢 /
脂质代谢 /
过氧化物酶体增殖物激活受体γ共激活因子1-α
Key words
milk tea /
glucose metabolism /
lipid metabolism /
PGC-1α
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