目的 研究乳源性多肽对新生儿抗感染作用,为生命早期健康成长提供营养保障。方法 利用质谱定量技术比较β-Casein-15肽在早产和足月产产妇母乳中的含量差异;通过Uniprot、SABLE、ProtParam tool在线数据库预测β-Casein-15肽生物学特征;以抑菌圈实验以及免疫荧光实验来检测β-Casein-15肽对大肠杆菌、小肠结肠炎菌、金黄色葡萄球菌的抑菌作用。结果 通过质谱分析以及生物信息学分析发现β-Casein-15肽的脂肪指数是122.33,亲水性是-0.387,不稳定系数是36.29。这些数据表明β-Casein-15肽是稳定性疏水性多肽。质谱定量分析发现β-Casein-15肽在早产儿产妇母乳中含量明显高于足月儿产妇,抑菌实验发现β-Casein-15肽对大肠杆菌、小肠结肠炎菌以及金黄色葡萄球菌生长均有明显的抑制作用。结论 这些结果提示β-Casein-15肽在抗新生儿感染中发挥着重要的作用。
Abstract
Objective To study the human milk resource peptide in the role of resistance of neonatal infection. Methods The content of β-Casein-15 in human milk was mesured by mass spectrometry technology.The biologic characteristics of β-Casein-15 peptide were predicted by Uniprot,SABLE,ProtParam tool and other online databases. Results The online databases showed that aliphatic index,grand average of hydropathicity and instability coefficient of β-Casein-15 were 122.33,-0.387 and 36.29,respectively.It indicated that β-Casein-15 was a hydrophobic stable peptide.And then,the content of β-Casein-15 was significantly decreased in premature compared to full term infants,detected by mass spectrometry technology.Moreover,the bacteriostasis experiment was found β-Casein-15 peptide in e.coli,enterocolitis bacteria and staphylococcus aureus had obvious bacteriostatic action. Conclusion These results indicate β-Casein-15 may play a critial role in promoting neonatal anti-infection.
关键词
母乳 /
β-Casein-15肽 /
抑菌
Key words
human milk /
β-Casein-15 peptide /
bacteriostatic
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基金
国家973计划项目(2013CB530604);国家自然科学基金(81330067;81601333;81670773)
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