目的 探讨新生儿缺氧缺血性脑病血清中 miRNA- 21、缺氧诱导因 子1(HIF-1α)的表达及临床意义,为临床上早期诊断及治疗缺氧缺血性脑病提供科学依据。方法 选取2012年1月-2013年6月在新乡市中心医院和妇幼保健院出生的新生儿,分为:缺血缺氧组49例;正常对照组29例,共计78 例。两组于生后 24~48 h 内采血5 mL。应用实时荧光定量 PCR 技术检测血清中 miRNA-21和HIF-1α的表达水平。结果 缺氧缺血组与对照组相比,缺氧缺血组血清中miRNA-21和HIF-1α均呈现高表达(P＜0.05)。结论 血清中miRNA-21和HIF-1α的高表达与缺氧缺血性脑病有关,并可作为早期判断脑损伤的有用的生物学指标。
Abstract
Objective To study the expression and significance of serum miRNA-21 and hypoxia inducible factor-1α (HIF-α) in newborns with asphyxia. Methods A total of 78 newborn infants were recruited in the study,including 49 newborn infants with asphyxia and 29 cases without asphyxia.In 24~48 h after birth,the serum miRNA-21 and hypoxia inducible factor-1α (HIF-1α) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Result The levels of serum miRNA-21 and HIF-1α in newborn infants with asphyxia were high than those of control group (P<0.05). Conclusions Serum miRNA-21 plays a role on the hypoxic brain damage after asphyxia by acting on the target genes of HIF-1α.The serum expression of miRNA-21 can be used as a marker in the early diagnosis of hypoxic brain damage after asphyxia.
关键词
窒息 /
新生儿 /
微小RNA-21 /
缺氧诱导因子-1α /
实时荧光定量PCR
Key words
asphyxia /
newborn infant /
miRNA-21 /
hypoxia inducible factor-1α /
quantitative real-time polymerase chain reaction
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参考文献
[1] Filipowicz W,Bhattacharyya SN,Sonenberg N.Mechanisms of post-transcriptional regulation by microRNAs:are the answers in sight?[J].Nat Rev Genet,2008,9(2):102-114.
[2] 张煜,郭军.微小 RNA 与缺血性脑损伤[J].中国医学科学院学报,2012,34(4):418-421.
[3] Bianchi F,Nicassio F,Marzi M,et al.A serum circulating miRNA di-agnostic test to identify asymptomatic high-risk individuals with early stage lung cancer[J].EMBO Mol Med,2011,3(8):495-503.
[4] 朱小瑜,张谦慎.重新认识新生儿窒息的诊断问题[J].中国新生儿科杂志,2011,26(4):217-220.
[5] Low JA.Determining the contribution of asphyxia to brain damage in the neonate[J].J Obstet Gynaecol Res,2004,30(4):276-286.
[6] 李晓玲,徐金霞,王雪兰,等.缺氧缺血性脑病新生儿心肌酶谱水平的变化及其与窒息后脑损伤的关系[J].中国妇幼保健,2011,26(32):5017-5019.
[7] 戴启宇,杨廷桐,于芳芳.疾病标志物-血清microRNA的研究进展[J].实用医药杂志,2012,29(6):558-562.
[8] Zeng L,Liu J,Wang Y,et al.MicroRNA-210 as a novel blood biomarker in acute cerebral ischemia[J].Front Biosci (Elite Ed),2011,3:1265-1272.
[9] Hua YJ,Tang ZY,Tu K,et al.Identification and target prediction of miRNAs specifically expressed in rat neural tissue[J].BMC Genomics,2009,10:214-220.
[10] Dong S,Cheng Y,Yang J,et al.MicroRNA expression signature and the role of microRNA-21 in the early phase of acute myocardial infarction[J].J Biol Chem,2009,284:29514-29525.
[11] Roy S,Khanna S,Hussain SR,et al.MicroRNA expression in response to murine myocardial infarction:miR-21 regulates fibroblast metalloprotease-2 via phosphatase and tensin homologue[J].Cardiovasc Res,2009,82(1):21-29.
基金
河南省科技厅重点资助项目(082102310082)
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