目的 观察出生后早期持续暴露于音乐环境对大鼠成年后罹患高血压和发生不良心血管事件的倾向。方法 初生SD雄性乳鼠20只,出生24 h内即暴露于音乐环境(莫扎特《G小调第40号交响曲》),另设初生SD雄性乳鼠20只作为对照组,同条件饲养,不听音乐。大鼠5~8周时,采用无创性套尾法每日监测血压,8周末处死,扫描电镜观察主动脉内皮形态,ELISA法检测大鼠血清血管紧张素Ⅱ(AngⅡ)、C反应蛋白(CRP)、纤溶酶原激活物抑制剂-1(PAI-1)、血浆脑钠肽(BNP)。结果 听音乐组与对照组大鼠成年后的体重、心率无统计学差异,收缩压和平均动脉压有下降趋势,但无统计学意义。音乐组大鼠主动脉内皮形态更佳,无明显纤维等附着;对照组大鼠主动脉内皮虽基本正常,但细胞间连接部分丢失,内膜附着物较多。音乐组大鼠血清CRP、PAI-1显著降低(P＜0.05),AngⅡ、BNP有降低趋势,但两组比较,差异无统计学意义(P>0.05)。结论 出生后早期持续暴露于音乐环境对大鼠心血管系统发育的影响极为重大,成年后大鼠血压下降虽不显著,但其发生不良心血管事件的倾向已大幅度减低。
Abstract
Objective To observe the early continued exposure to music environment on blood pressure and the incidence of adverse cardiovascular events of rats in adulthood. Methods 20 male SD rats was exposed to music (Mozart 《G minor Symphony No.fortieth》) in 24h after birth,the same age SD rats as control group,keeping the same conditions but without music.In 5~8 weeks,blood pressure was monitored by noninvasive method daily.Afer 8 weeks,rats were sacrificed,aortic endothelial morphology was observed by scanning electron microscope and angiotensin II (Ang II),C reactive protein (CRP),plasminogen activator inhibitor -1 (PAI-1),plasma brain natriuretic peptide (BNP) in serum was determined by ELISA. Results The rats of music group and control had no significant differences in weight and heart rate.Systolic and mean arterial pressure decreased in rats of music group,but without statistical significance.The aortic endothelial morphology of rats of music group was better,with no fiber attachment;that of control group was also normal,but some of the cell connection was missing,and with more endometrial attachment.The serum CRP,PAI-1 of rats of music group decreased significantly (P<0.05).Ang Ⅱ,BNP had a decreasing tendency,but no significant differences were found between the two groups. Conclusion The early continuous exposure to music environment demonstrated beneficial effects on the cardiovascular system in developing rats.The decreasement of blood pressure of adult rats is not very significant,but its propensity of occurrence of adverse cardiovascular events had been greatly reduced.
关键词
音乐 /
不良心血管事件危险因素 /
血压
Key words
music /
risk of adverse cardiovascular events /
blood pressure
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基金
国家自然科学基金项目(81072962); 济南市科技局计划项目(201121032)
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